ko.cwru.edu
Targeting
http://ko.cwru.edu/services/targeting.shtml
What You Will Provide Part I. A map of the targeting strategy showing the selectable markers, arm lengths, and deletion length. The map should clearly indicate the linearization site. Arrange a meeting with us to discuss the targeting strategy. What We Will Do Part I. We will provide you with ES cell DNA. What You Will Provide Part II. An autoradiogram of a genomic Southern of ES cell genomic DNA cut with your diagnostic enzyme and probed with your external probe. What We Will Do Part II. You will cut th...
ko.cwru.edu
Transgenic Mice
http://ko.cwru.edu/services/transgenics.shtml
What You Will Provide Part I. 200 micrograms of pure plasmid DNA (e.g. Qiagen kit-purified) at a concentration of 1 µg/µl or greater, or BAC DNA prepared by this protocol. Information on the restriction enzymes which will allow us to cut and gel-purify a plasmid insert from the backbone. A photo of a gel with markers showing the plasmid insert liberated from the plasmid backbone by restriction digest with the enzymes we will use to gel-purify the insert. What We Will Do. We will gel-purify plasmid inserts.
knockout.cwru.edu
Transgenic or Knockout Mouse?
http://knockout.cwru.edu/info/strategies.html
Transgenic or Knockout Mouse? Strategic Decisions: Gene Targeted or Transgenic Model? Excerpted and modified from Conlon RA (2011) Neuromethods: Transgenic and Gene Targeted Models of Dementia. Springer Science. Transgenics and Gene Targeting. Genetic Mechanism of Disease and Choice of Technologies. If a disease results from complete loss of function (a null mutation), then the gene can be inactivated by gene targeting (gene knockout). Both homozygous recessive traits (both gene copies inactivated by...
knockout.cwru.edu
Transgenic Mice
http://knockout.cwru.edu/services/transgenics.shtml
What You Will Provide Part I. 200 micrograms of pure plasmid DNA (e.g. Qiagen kit-purified) at a concentration of 1 µg/µl or greater, or BAC DNA prepared by this protocol. Information on the restriction enzymes which will allow us to cut and gel-purify a plasmid insert from the backbone. A photo of a gel with markers showing the plasmid insert liberated from the plasmid backbone by restriction digest with the enzymes we will use to gel-purify the insert. What We Will Do. We will gel-purify plasmid inserts.
knockout.cwru.edu
The Mouse as a Model System
http://knockout.cwru.edu/info/mousemodel.html
The Mouse as a Model System. The Mouse as a Model System. How to Breed Mice. The mouse is a popular model system because it is a mammal with sophisticated genetic tools and significant genetic resources. Adult mice weigh 30-40 grams (50,000 to 70,000 grams for a young adult human) have a blood volume of 2 ml (4,800 ml for humans), and a resting heart rate of 500-700 bpm (60-80 bpm for humans). Laboratory mice are unique in that there are a large number (hundreds) of inbred strains. Inbred strains are...
pacmanfly.org
P[acman] Resources - Reagents
http://www.pacmanfly.org/reagents.html
Available from the Drosophila. Available from other sources. Available from BACPAC Resources. Available from the BDSC: see stocks page. Please send comments or questions about the website to Karen Schulze.
geno-gtac.co.jp
世界汎用BAC/PACクローン～有限会社ジェノテックス～
http://www.geno-gtac.co.jp/BAC_clone.html
ゲノムマーカー BAC/PAC クローン の販売. 大腸菌人工染色体 Bacterial Artificial Chromosome BAC によるゲノムライブラリーのうち、ヒトRPCI-11とマウスRP-23 BACライブラリーは、ゲノムプロジェクトの共通材料として汎用されたために、ライブラリーのクローン番号から何番染色体のどの位置にあるのか、どの遺伝子がどのように配置されているのかが、NCBIデータベースで検索することができます。 1997年、 ローズウェル癌研究所 Roswell Park Cancer Institute は、米国NIHの依頼でヒト全ゲノムシークエンス用としてRP-11を作製しました。 ヒト男性RP-1, 3, 4 and 5 PACライブラリー. C57BL/6J系統のマウス メス の腎臓,、および脳から抽出さしたDNAを制限酵素EcoR で部分分解し、pBACe3.6ベクターに挿入、大腸菌DH10Bでクローニングしました。 1)マップ ドラフトシークエンス http:/ www.ncbi.nlm.nih.gov/. RP-1,3,4,5PAC.
flyrnai.org
Find Dmel dsRNAs and Drosophila Species Genes for RNAi Rescue
http://www.flyrnai.org/cgi-bin/RNAi_find_rescue_compl.pl
Find Dmel dsRNAs and Drosophila Species Genes for RNAi Rescue. Enter a Dmel Gene Symbol, FBgn, CG or DRSC ID:. Select Species to Search:. Select D. melanogaster. Select Orthologous Gene Match Length Threshold:. You can use this tool to find Drosophila. Clones are available from the DRSC. Clones are available from BACPAC. Clones are available from DGRC. For successful RNAi rescue, it is also important to use a dsRNA that does not suppress the rescue transgenes derived from a Drosophila.
ko.case.edu
Targeting
http://ko.case.edu/services/targeting.shtml
What You Will Provide Part I. A map of the targeting strategy showing the selectable markers, arm lengths, and deletion length. The map should clearly indicate the linearization site. Arrange a meeting with us to discuss the targeting strategy. What We Will Do Part I. We will provide you with ES cell DNA. What You Will Provide Part II. An autoradiogram of a genomic Southern of ES cell genomic DNA cut with your diagnostic enzyme and probed with your external probe. What We Will Do Part II. You will cut th...
ko.case.edu
Transgenic Mice
http://ko.case.edu/services/transgenics.shtml
What You Will Provide Part I. 200 micrograms of pure plasmid DNA (e.g. Qiagen kit-purified) at a concentration of 1 µg/µl or greater, or BAC DNA prepared by this protocol. Information on the restriction enzymes which will allow us to cut and gel-purify a plasmid insert from the backbone. A photo of a gel with markers showing the plasmid insert liberated from the plasmid backbone by restriction digest with the enzymes we will use to gel-purify the insert. What We Will Do. We will gel-purify plasmid inserts.
