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CRISPR-P

CRISPR-P: A web tool for synthetic single-guide RNA design of CRISPR-system in plants. CRISPR-Cas system in application (modified from. Charpentier and Doudna 2013. Yang Lei#, Li Lu#, Hai-Yang Liu, Sen L, Feng Xing, Ling-Ling Chen*. CRISPR-P: A web tool for synthetic single-guide RNA design of CRISPR-system in plants. Mol Plant, 2014, 7(9): 1494-1496. doi: 10.1093/mp/ssu044. Version CRISPR-P v1.0, released March 27th, 2014. Author: BioInfo.

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CRISPR-P | cbi.hzau.edu.cn Reviews
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CRISPR-P: A web tool for synthetic single-guide RNA design of CRISPR-system in plants. CRISPR-Cas system in application (modified from. Charpentier and Doudna 2013. Yang Lei#, Li Lu#, Hai-Yang Liu, Sen L, Feng Xing, Ling-Ling Chen*. CRISPR-P: A web tool for synthetic single-guide RNA design of CRISPR-system in plants. Mol Plant, 2014, 7(9): 1494-1496. doi: 10.1093/mp/ssu044. Version CRISPR-P v1.0, released March 27th, 2014. Author: BioInfo.
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CRISPR-P | cbi.hzau.edu.cn Reviews

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CRISPR-P: A web tool for synthetic single-guide RNA design of CRISPR-system in plants. CRISPR-Cas system in application (modified from. Charpentier and Doudna 2013. Yang Lei#, Li Lu#, Hai-Yang Liu, Sen L, Feng Xing, Ling-Ling Chen*. CRISPR-P: A web tool for synthetic single-guide RNA design of CRISPR-system in plants. Mol Plant, 2014, 7(9): 1494-1496. doi: 10.1093/mp/ssu044. Version CRISPR-P v1.0, released March 27th, 2014. Author: BioInfo.

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CRISPR-P

http://cbi.hzau.edu.cn/crispr/help.php

This section provides the basic knowledge of CRISPR-Cas9. Epeats) is an acronym for DNA loci that contain multiple, short, direct repetitions of base sequences. Each repetition contains a series of base pairs followed by the same or a similar series in reverse and then by 30 or so base pairs known as spacer DNA. CRISPRs are found in the genomes of approximately 40% of sequenced bacteria and 90% of sequenced archaea (Grissa. CRISPRs are often associated with. Marraffini and Sontheimer, 2010). Characterize...

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CRISPR-P

http://cbi.hzau.edu.cn/crispr/news.php

ClusterMaps was reloaded because previous map service crashed. ClustrMaps and new plant organisms were added. Now 33 genomes are included in CRISPR-P. Add "Nicotiana benthamiana", "Lotus japonicus" to the target genomes. Many plant organisms are added, and some remove. Now 26 genomes are included in CRISPR-P. Update enzyme information form REBASE. Chose "Oryza sativa ssp. japonica cv. Nipponbare" genome as the test genome. And later ZS97, MH63 were added. Version CRISPR-P v1.0, you are the 184167.

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CRISPR-P

http://cbi.hzau.edu.cn/cgi-bin/CRISPR

Arabidopsis lyrata (v.1.0). Brachypodium distachyon (v1.0). Brassica napus (v4.1). Brassica oleracea (v1.0). Capsella rubella(v1.0 ). Chlamydomonas reinhardtii (v5.5). Citrullus lanatus (v1.0). Citrus sinensis (v2.0). Cucumis melo (v3.5). Fragaria vesca(v2.0.a1). Glycine max (V1.0). Lotus japonicus (v3.0). Medicago truncatula (Mt4.0v2). Nicotiana benthamiana (v0.4.4). Oryza brachyantha (v1.4b). Oryza glaberrima (AGI1.1). Populus trichocarpa (JGI2.0). Selaginella moellendorffii (v1.0).

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LEGUME LABORATORY – Page 2 – "You can't build a peaceful world on empty stomachs and human misery" – Norman Borlaug

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You can't build a peaceful world on empty stomachs and human misery – Norman Borlaug. CRISPR Efficiency and Specificity in Arabidopsis. October 19, 2016. Related to our recent post about how we identify genes related to traits. A recent PLoS One research article. Looked at the use of CRISPR/Cas9 to target multiple sites in one transformation event in. Particularly, they looked at its efficiency transforming a number of genes and also assessed the rate of off-target modifications made. For each of the sev...

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CRISPR-P

CRISPR-P: A web tool for synthetic single-guide RNA design of CRISPR-system in plants. CRISPR-Cas system in application (modified from. Charpentier and Doudna 2013. Yang Lei#, Li Lu#, Hai-Yang Liu, Sen L, Feng Xing, Ling-Ling Chen*. CRISPR-P: A web tool for synthetic single-guide RNA design of CRISPR-system in plants. Mol Plant, 2014, 7(9): 1494-1496. doi: 10.1093/mp/ssu044. Version CRISPR-P v1.0, released March 27th, 2014. Author: BioInfo.

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